Skip to content Skip to sidebar Skip to footer

Alt.com reviews

Overall performance were expressed due to the fact optical density at the 450 nm (OD

Overall performance were expressed due to the fact optical density at the 450 nm (OD

Heparinised blood samples from all groups of animals were stored overnight at room temperature before stimulation (dos50 µl in duplicate wells of a 96-well plate) for 20 to 24 h at 37 °C in 5% CO2 with MDT and DST reagents at a final assay concentration of 0.1 µg/ml for each protein/peptide component. As positive and negative controls, blood samples were cultured with pokeweed mitogen (10 µg/ml; Sigma, UK) and RPMI-1640 alone (Gibco, UK) respectively. After stimulation, blood was centrifuged at 300 g for 10 min and the plasma supernatant was harvested and stored at ? 80 °C until required.